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Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform
In this paper, we describe the stepwise development of a cell-free protein synthesis (CFPS) platform derived from cultured Chinese hamster ovary (CHO) cells. We provide a retrospective summary of the design challenges we faced, and the optimized methods developed for the cultivation of cells and the...
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| Veröffentlicht in: | Front Bioeng Biotechnol |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Frontiers Media S.A.
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7884609/ https://ncbi.nlm.nih.gov/pubmed/33604330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.604091 |
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