Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains

This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expres...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Chen, Chuan, Sun, Zehua, Liu, Xianglei, Li, Wei, Dimitrov, Dimiter S.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8164376/
https://ncbi.nlm.nih.gov/pubmed/34095859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100617
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