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Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expres...
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| Gepubliceerd in: | STAR Protoc |
|---|---|
| Hoofdauteurs: | , , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Elsevier
2021
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8164376/ https://ncbi.nlm.nih.gov/pubmed/34095859 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100617 |
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