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Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides

Pairs of unnatural nucleotides are used to expand the genetic code and create artificial DNA or RNA templates. In general, an approach is used to engineer orthogonal systems capable of reading codons comprising artificial nucleotides; however, DNA and RNA polymerases capable of recognizing unnatural...

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Bibliografiske detaljer
Udgivet i:Int J Mol Sci
Main Authors: Egorova, Tatiana, Shuvalova, Ekaterina, Mukba, Sabina, Shuvalov, Alexey, Kolosov, Peter, Alkalaeva, Elena
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8155940/
https://ncbi.nlm.nih.gov/pubmed/34069057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22105186
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