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Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides

Pairs of unnatural nucleotides are used to expand the genetic code and create artificial DNA or RNA templates. In general, an approach is used to engineer orthogonal systems capable of reading codons comprising artificial nucleotides; however, DNA and RNA polymerases capable of recognizing unnatural...

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書目詳細資料
發表在:Int J Mol Sci
Main Authors: Egorova, Tatiana, Shuvalova, Ekaterina, Mukba, Sabina, Shuvalov, Alexey, Kolosov, Peter, Alkalaeva, Elena
格式: Artigo
語言:Inglês
出版: MDPI 2021
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC8155940/
https://ncbi.nlm.nih.gov/pubmed/34069057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22105186
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