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A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets
Transcriptional changes happen within minutes; however, RNAi or genetic deletion requires days to weeks before transcription networks can be analyzed. This limitation has made it challenging to distinguish direct from indirect targets of sequence-specific transcription factors. This inability to def...
Shranjeno v:
| izdano v: | STAR Protoc |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2021
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8142277/ https://ncbi.nlm.nih.gov/pubmed/34041503 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100530 |
| Oznake: |
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