Protocol for analyzing the biosynthesis and degradation of N-glycan precursors in mammalian cells

N-glycosylation is a fundamental post-translational protein modification in the endoplasmic reticulum of eukaryotic cells. The biosynthetic and catabolic flux of N-glycans in eukaryotic cells has long been analyzed by metabolic labeling using radiolabeled sugars. Here, we introduce a non-radiolabeli...

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Podrobná bibliografie
Vydáno v:STAR Protoc
Hlavní autoři: Harada, Yoichiro, Nakajima, Kazuki, Li, Shengtao, Suzuki, Tadashi, Taniguchi, Naoyuki
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2021
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7890039/
https://ncbi.nlm.nih.gov/pubmed/33659899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100316
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