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Protocol for analyzing the biosynthesis and degradation of N-glycan precursors in mammalian cells
N-glycosylation is a fundamental post-translational protein modification in the endoplasmic reticulum of eukaryotic cells. The biosynthetic and catabolic flux of N-glycans in eukaryotic cells has long been analyzed by metabolic labeling using radiolabeled sugars. Here, we introduce a non-radiolabeli...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7890039/ https://ncbi.nlm.nih.gov/pubmed/33659899 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100316 |
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