Immunofluorescence microscopy-based assessment of cytosolic DNA accumulation in mammalian cells

Here, we describe an immunofluorescence (IF) microscopy-based approach to quantify cytosolic double-stranded DNA molecules in cultured eukaryotic cells upon the selective and specific permeabilization of plasma membranes. This technique is compatible with widefield microscopy coupled with automated...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:STAR Protoc
Asıl Yazarlar: Sato, Ai, Buque, Aitziber, Yamazaki, Takahiro, Bloy, Norma, Petroni, Giulia, Galluzzi, Lorenzo
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2021
Konular:
Protocol
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC8141942/
https://ncbi.nlm.nih.gov/pubmed/34041502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100488
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