Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing

Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies. However, human bone marrow stromal cells are difficult to isolate and prone to damage during isolation. This pro...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Gleitz, Hélène F.E., Snoeren, Inge A.M., Fuchs, Stijn N.R., Leimkühler, Nils B., Schneider, Rebekka K.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8134068/
https://ncbi.nlm.nih.gov/pubmed/34027494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100538
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