Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy

This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, na...

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Sparad:
Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Lu, Yan, Wang, Fangfang, Wang, Haoyu, Bastians, Philipp, Hua, Yunfeng
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2021
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC8121772/
https://ncbi.nlm.nih.gov/pubmed/34027478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100515
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