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Rapid and efficient enhancer cloning and in vivo screening using the developing chick embryo
Here, we describe a highly efficient, medium-throughput strategy for cloning and in vivo screening of putative enhancers using the chick embryo. By incorporating 48 unique nanotags for use in NanoString nCounter® across three different fluorescent reporters and developing a rapid and efficient diges...
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| Udgivet i: | STAR Protoc |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8121703/ https://ncbi.nlm.nih.gov/pubmed/34027475 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100507 |
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