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Rapid and efficient enhancer cloning and in vivo screening using the developing chick embryo

Here, we describe a highly efficient, medium-throughput strategy for cloning and in vivo screening of putative enhancers using the chick embryo. By incorporating 48 unique nanotags for use in NanoString nCounter® across three different fluorescent reporters and developing a rapid and efficient diges...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Williams, Ruth M., Sauka-Spengler, Tatjana
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8121703/
https://ncbi.nlm.nih.gov/pubmed/34027475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100507
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