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Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo

CRISPR/Cas9 genome engineering has revolutionised all aspects of biological research, with epigenome engineering transforming gene regulation studies. Here, we present an optimised, adaptable toolkit enabling genome and epigenome engineering in the chicken embryo, and demonstrate its utility by prob...

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Detalhes bibliográficos
Publicado no:Development
Main Authors: Williams, Ruth M., Senanayake, Upeka, Artibani, Mara, Taylor, Gunes, Wells, Daniel, Ahmed, Ahmed Ashour, Sauka-Spengler, Tatjana
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists Ltd 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5869010/
https://ncbi.nlm.nih.gov/pubmed/29386245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.160333
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