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Dissociation of chick embryonic tissue for FACS and preparation of isolated cells for genome-wide downstream assays
In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an optimized...
Shranjeno v:
| izdano v: | STAR Protoc |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2021
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8039852/ https://ncbi.nlm.nih.gov/pubmed/33870222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100414 |
| Oznake: |
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