Dissociation of chick embryonic tissue for FACS and preparation of isolated cells for genome-wide downstream assays

In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an optimized...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Williams, Ruth M., Sauka-Spengler, Tatjana
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8039852/
https://ncbi.nlm.nih.gov/pubmed/33870222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100414
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