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Active-Site Models of Streptococcus pyogenes Cas9 in DNA Cleavage State
CRISPR-Cas9 is a powerful tool for target genome editing in living cells. Significant advances have been made to understand how this system cleaves target DNA. However, due to difficulty in determining active CRISPR-Cas9 structure in DNA cleavage state by X-ray and cryo-EM, it remains uncertain how...
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| Veröffentlicht in: | Front Mol Biosci |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Frontiers Media S.A.
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8112549/ https://ncbi.nlm.nih.gov/pubmed/33987202 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2021.653262 |
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