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Active-Site Models of Streptococcus pyogenes Cas9 in DNA Cleavage State

CRISPR-Cas9 is a powerful tool for target genome editing in living cells. Significant advances have been made to understand how this system cleaves target DNA. However, due to difficulty in determining active CRISPR-Cas9 structure in DNA cleavage state by X-ray and cryo-EM, it remains uncertain how...

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Bibliografiske detaljer
Udgivet i:Front Mol Biosci
Main Authors: Tang, Honghai, Yuan, Hui, Du, Wenhao, Li, Gan, Xue, Dongmei, Huang, Qiang
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8112549/
https://ncbi.nlm.nih.gov/pubmed/33987202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2021.653262
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