A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

Crosslinking and immunoprecipitation (CLIP) methods are powerful techniques to interrogate direct protein-RNA interactions and dissect posttranscriptional gene regulatory networks. One widely used CLIP variant is photoactivatable ribonucleoside enhanced CLIP (PAR-CLIP) that involves in vivo labeling...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Anastasakis, Dimitrios G, Jacob, Alexis, Konstantinidou, Parthena, Meguro, Kazuyuki, Claypool, Duncan, Cekan, Pavol, Haase, Astrid D, Hafner, Markus
Format: Artigo
Language:Inglês
Published: Oxford University Press 2021
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8096255/
https://ncbi.nlm.nih.gov/pubmed/33503264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab011
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