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Simultaneous Directed Evolution of Coupled Enzymes for Efficient Asymmetric Synthesis of l-Phosphinothricin

The traditional strategy to improve the efficiency of an entire coupled-enzyme system relies on the separate direction of the evolution of enzymes involved in their respective enzymatic reactions. This strategy can lead to enhanced single-enzyme catalytic efficiency but may also lead to a loss of co...

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Publicado en:Appl Environ Microbiol
Autores principales: Cheng, Feng, Li, Qing-Hua, Zhang, Hua-Yue, Wei, Lan, Zhang, Jia-Min, Li, Ju-Mou, Xue, Ya-Ping, Zheng, Yu-Guo
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2021
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8090864/
https://ncbi.nlm.nih.gov/pubmed/33310717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02563-20
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