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High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for larg...
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| 發表在: | STAR Protoc |
|---|---|
| Main Authors: | , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2021
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8086141/ https://ncbi.nlm.nih.gov/pubmed/33997803 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100463 |
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