High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells

Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for larg...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:STAR Protoc
Autori principali: Sarrafha, Lily, Parfitt, Gustavo M., Reyes, Ricardo, Goldman, Camille, Coccia, Elena, Kareva, Tatyana, Ahfeldt, Tim
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2021
Soggetti:
Protocol
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086141/
https://ncbi.nlm.nih.gov/pubmed/33997803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100463
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