Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein

Glutathione S-transferase (GST) from Schistosoma japonicum has been widely used as a tag for affinity purification and pulldown of fusion proteins to detect protein–protein interactions. However, the reliability of this technique is undermined by the formation of GST-fused protein aggregates after i...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Exp Mol Med
Prif Awduron: Kim, Hyo-Jun, Lee, Jin-Haeng, Lee, Ki Baek, Shin, Ji-Woong, Kwon, Mee-ae, Lee, Soojin, Jeong, Eui Man, Cho, Sung-Yup, Kim, In-Gyu
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Publishing Group UK 2021
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8080825/
https://ncbi.nlm.nih.gov/pubmed/33441971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s12276-020-00549-9
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