Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein

Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin frequency is low compared with deletion induction. Althoug...

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Udgivet i:Stem Cell Reports
Main Authors: Kagita, Akihiro, Lung, Mandy S.Y., Xu, Huaigeng, Kita, Yuto, Sasakawa, Noriko, Iguchi, Takahiro, Ono, Miyuki, Wang, Xiou H., Gee, Peter, Hotta, Akitsu
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8072016/
https://ncbi.nlm.nih.gov/pubmed/33711268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2021.02.013
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