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Expanding the Scope of RNA Metabolic Labeling with Vinyl Nucleosides and Inverse Electron-Demand Diels-Alder Chemistry

Optimized and stringent chemical methods to profile nascent RNA expression are still in demand. Herein, we expand the toolkit for metabolic labeling of RNA through application of inverse electron demand Diels-Alder (IEDDA) chemistry. Structural examination of metabolic enzymes guided the design and...

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Detalhes bibliográficos
Publicado no:ACS Chem Biol
Main Authors: Kubota, Miles, Nainar, Sarah, Parker, Shane M., England, Whitney, Furche, Filipp, Spitale, Robert C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8061575/
https://ncbi.nlm.nih.gov/pubmed/31310712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.9b00079
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