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Mapping DNA Breaks by Next-Generation Sequencing

Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topois...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Baranello, Laura, Kouzine, Fedor, Wojtowicz, Damian, Cui, Kairong, Zhao, Keji, Przytycka, Teresa M., Capranico, Giovanni, Levens, David
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8057127/
https://ncbi.nlm.nih.gov/pubmed/29043624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7306-4_13
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