Nucleotide-resolution DNA double-strand breaks mapping by next-generation sequencing

We present a genome-wide method to map DNA double-strand breaks (DSBs) at nucleotide resolution by direct in situ breaks labeling, enrichment on streptavidin, and next-generation sequencing (BLESS). We comprehensively validated and tested BLESS using different human and mouse cells, DSBs-inducing ag...

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Detalhes bibliográficos
Main Authors: Crosetto, Nicola, Mitra, Abhishek, Silva, Maria Joao, Bienko, Magda, Dojer, Norbert, Wang, Qi, Karaca, Elif, Chiarle, Roberto, Skrzypczak, Magdalena, Ginalski, Krzysztof, Pasero, Philippe, Rowicka, Maga, Dikic, Ivan
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3651036/
https://ncbi.nlm.nih.gov/pubmed/23503052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2408
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