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RecJ 5′ Exonuclease Digestion of Oligonucleotide Failure Strands: A “Green” Method of Trityl-On Purification

Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, g...

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Bibliographic Details
Published in:Biochemistry
Main Authors: Jensen, Michael, Davis, Ronald
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8045232/
https://ncbi.nlm.nih.gov/pubmed/28459543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00010
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