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RecJ 5′ Exonuclease Digestion of Oligonucleotide Failure Strands: A “Green” Method of Trityl-On Purification
Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, g...
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| Publié dans: | Biochemistry |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8045232/ https://ncbi.nlm.nih.gov/pubmed/28459543 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00010 |
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