Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melted initiat...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:STAR Protoc
Prif Awduron: Martinez, Sergio E., Singh, Anupam, De Wijngaert, Brent, Sultana, Shemaila, Dharia, Chhaya, Vanbuel, Hans, Shen, Jiayu, Vasilchuk, Daniel, Patel, Smita S., Das, Kalyan
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Elsevier 2021
Pynciau:
Protocol
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8044712/
https://ncbi.nlm.nih.gov/pubmed/33870232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100431
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