Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melted initiat...

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Dades bibliogràfiques
Publicat a:STAR Protoc
Autors principals: Martinez, Sergio E., Singh, Anupam, De Wijngaert, Brent, Sultana, Shemaila, Dharia, Chhaya, Vanbuel, Hans, Shen, Jiayu, Vasilchuk, Daniel, Patel, Smita S., Das, Kalyan
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Protocol
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8044712/
https://ncbi.nlm.nih.gov/pubmed/33870232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100431
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