Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melted initiat...

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Podrobná bibliografie
Vydáno v:STAR Protoc
Hlavní autoři: Martinez, Sergio E., Singh, Anupam, De Wijngaert, Brent, Sultana, Shemaila, Dharia, Chhaya, Vanbuel, Hans, Shen, Jiayu, Vasilchuk, Daniel, Patel, Smita S., Das, Kalyan
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2021
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8044712/
https://ncbi.nlm.nih.gov/pubmed/33870232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100431
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