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Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells

More than 30% of the total amount of proteins synthesized in mammalian cells follow the secretory pathway in order to mature and be properly sorted to their final destinations. Among several methodologies that describe live-cell monitoring of vesicles, the Retention Using Selective Hooks (RUSH) syst...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Pakdel, Mehrshad, Pacheco-Fernandez, Natalia, von Blume, Julia
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8032492/
https://ncbi.nlm.nih.gov/pubmed/33855118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3958
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