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Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells

More than 30% of the total amount of proteins synthesized in mammalian cells follow the secretory pathway in order to mature and be properly sorted to their final destinations. Among several methodologies that describe live-cell monitoring of vesicles, the Retention Using Selective Hooks (RUSH) syst...

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Podrobná bibliografie
Vydáno v:Bio Protoc
Hlavní autoři: Pakdel, Mehrshad, Pacheco-Fernandez, Natalia, von Blume, Julia
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8032492/
https://ncbi.nlm.nih.gov/pubmed/33855118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3958
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