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Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells

More than 30% of the total amount of proteins synthesized in mammalian cells follow the secretory pathway in order to mature and be properly sorted to their final destinations. Among several methodologies that describe live-cell monitoring of vesicles, the Retention Using Selective Hooks (RUSH) syst...

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Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Pakdel, Mehrshad, Pacheco-Fernandez, Natalia, von Blume, Julia
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8032492/
https://ncbi.nlm.nih.gov/pubmed/33855118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3958
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