CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

hTERT-RPE1 cells are genetically stable near diploid cells widely used to model cell division, DNA repair, or ciliogenesis in a non-transformed context. However, poor transfectability and limited homology-directed repair capacity hamper their amenability to gene editing. Here, we describe a protocol...

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Vydáno v:STAR Protoc
Hlavní autoři: Ghetti, Sabrina, Burigotto, Matteo, Mattivi, Alessia, Magnani, Giovanni, Casini, Antonio, Bianchi, Andrea, Cereseto, Anna, Fava, Luca L.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2021
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8025146/
https://ncbi.nlm.nih.gov/pubmed/33855309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100407
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