POT1 stability and binding measured by fluorescence thermal shift assays

The protein POT1 (Protection of Telomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end fusions. It is the only component of shelterin that binds single-stranded DNA. We describe here the application of two separate fluorescent th...

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Publicado en:PLoS One
Autores principales: DeLeeuw, Lynn W., Monsen, Robert C., Petrauskas, Vytautas, Gray, Robert D., Baranauskiene, Lina, Matulis, Daumantas, Trent, John O., Chaires, Jonathan B.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8009405/
https://ncbi.nlm.nih.gov/pubmed/33784306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0245675
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