Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

The analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90αN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII)...

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Detalhes bibliográficos
Main Authors: Zubrienė, Asta, Matulienė, Jurgita, Baranauskienė, Lina, Jachno, Jelena, Torresan, Jolanta, Michailovienė, Vilma, Cimmperman, Piotras, Matulis, Daumantas
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2705510/
https://ncbi.nlm.nih.gov/pubmed/19582223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms10062662
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