Live-cell FLIM-FRET using a commercially available system.

Förster resonance energy transfer (FRET)-based sensors have been powerful tools in cell biologists’ toolkit for decades. Informed by fundamental understanding of fluorescent proteins, protein-protein interactions, and the structural biology of reporter components, researchers have been able to emplo...

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發表在:Methods Cell Biol
Main Authors: Castellani, Colleen M., Torres-Ocampo, Ana P., Breffke, Jens, White, Adam B., Chambers, James J., Stratton, Margaret M., Maresca, Thomas J.
格式: Artigo
語言:Inglês
出版: 2020
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC8006575/
https://ncbi.nlm.nih.gov/pubmed/32423651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2020.02.002
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