Adenovirus Type-5 Entry and Disassembly Followed in Living Cells by FRET, Fluorescence Anisotropy, and FLIM

We have used fluorescence resonance energy transfer (FRET) to follow the process of capsid disassembly for adenovirus (Ad) serotype 5 (Ad5) in living CHO-CAR cells. Ad5 were weakly labeled on their capsid proteins with FRET donor and acceptor fluorophores. A progressive decrease in FRET efficiency r...

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Autors principals: Martin-Fernandez, Marisa, Longshaw, Samantha V., Kirby, Ian, Santis, George, Tobin, Mark J., Clarke, David T., Jones, Gareth R.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2004
Matèries:
Spectroscopy, Imaging, Other Techniques
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304470/
https://ncbi.nlm.nih.gov/pubmed/15298934
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.103.035444
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