CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriopha...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Wetzel, Katherine S., Guerrero-Bustamante, Carlos A., Dedrick, Rebekah M., Ko, Ching-Chung, Freeman, Krista G., Aull, Haley G., Divens, Ashley M., Rock, Jeremy M., Zack, Kira M., Hatfull, Graham F.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7990910/
https://ncbi.nlm.nih.gov/pubmed/33762639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-86112-6
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