CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriopha...

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Vydáno v:Sci Rep
Hlavní autoři: Wetzel, Katherine S., Guerrero-Bustamante, Carlos A., Dedrick, Rebekah M., Ko, Ching-Chung, Freeman, Krista G., Aull, Haley G., Divens, Ashley M., Rock, Jeremy M., Zack, Kira M., Hatfull, Graham F.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2021
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7990910/
https://ncbi.nlm.nih.gov/pubmed/33762639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-86112-6
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