CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriopha...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wetzel, Katherine S., Guerrero-Bustamante, Carlos A., Dedrick, Rebekah M., Ko, Ching-Chung, Freeman, Krista G., Aull, Haley G., Divens, Ashley M., Rock, Jeremy M., Zack, Kira M., Hatfull, Graham F.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7990910/
https://ncbi.nlm.nih.gov/pubmed/33762639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-86112-6
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