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Live imaging and quantitation of nascent transcription using the MS2/MCP system in the Drosophila embryo
Visualizing transcription live in Drosophila is providing important new insights into the spatiotemporal regulation of transcription. Here, we describe a protocol to visualize and quantitate transcription from gene loci that are tagged with MS2 stem-loop sequences in the Drosophila embryo. MS2 stem-...
Gardado en:
| Publicado en: | STAR Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7982776/ https://ncbi.nlm.nih.gov/pubmed/33778778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100379 |
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