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Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames

Upstream open reading frame (uORF) translation disrupts scanning 43S flux on mRNA and modulates main open reading frame (mORF) translation efficiency. Current tools, however, have limited access to ribosome dynamics in both upstream and main ORFs of an mRNA. Here, we develop a new two-color in vitro...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Gaba, Anthony, Wang, Hongyun, Fortune, Trinisia, Qu, Xiaohui
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7969011/
https://ncbi.nlm.nih.gov/pubmed/33330921
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1185
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