In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region

We report a systematic combinatorial exploration of affinity enhancement of antibodies by insertions and deletions (InDels). Transposon-based introduction of InDels via the method TRIAD (transposition-based random insertion and deletion mutagenesis) was used to generate large libraries with random i...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Skamaki, Kalliopi, Emond, Stephane, Chodorge, Matthieu, Andrews, John, Rees, D. Gareth, Cannon, Daniel, Popovic, Bojana, Buchanan, Andrew, Minter, Ralph R., Hollfelder, Florian
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2020
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7959553/
https://ncbi.nlm.nih.gov/pubmed/33067389
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2002954117
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki