Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIA...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Emond, Stephane, Petek, Maya, Kay, Emily J., Heames, Brennen, Devenish, Sean R. A., Tokuriki, Nobuhiko, Hollfelder, Florian
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7351745/
https://ncbi.nlm.nih.gov/pubmed/32651386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-17061-3
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!

Lignende værker