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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIA...
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| Publicat a: | Nat Commun |
|---|---|
| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group UK
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7351745/ https://ncbi.nlm.nih.gov/pubmed/32651386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-17061-3 |
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