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Probing the Role of the Conserved Arg174 in Formate Dehydrogenase by Chemical Modification and Site-Directed Mutagenesis
The reactive adenosine derivative, adenosine 5′-O-[S-(4-hydroxy-2,3-dioxobutyl)]-thiophosphate (AMPS-HDB), contains a dicarbonyl group linked to the purine nucleotide at a position equivalent to the pyrophosphate region of NAD(+). AMPS-HDB was used as a chemical label towards Candida boidinii format...
Enregistré dans:
| Publié dans: | Molecules |
|---|---|
| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MDPI
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7956174/ https://ncbi.nlm.nih.gov/pubmed/33668802 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules26051222 |
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