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Probing the Role of the Conserved Arg174 in Formate Dehydrogenase by Chemical Modification and Site-Directed Mutagenesis

The reactive adenosine derivative, adenosine 5′-O-[S-(4-hydroxy-2,3-dioxobutyl)]-thiophosphate (AMPS-HDB), contains a dicarbonyl group linked to the purine nucleotide at a position equivalent to the pyrophosphate region of NAD(+). AMPS-HDB was used as a chemical label towards Candida boidinii format...

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Vydáno v:Molecules
Hlavní autoři: Alqarni, Mohammed Hamed, Foudah, Ahmed Ibrahim, Muharram, Magdy Mohamed, Budurian, Haritium, Labrou, Nikolaos E.
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7956174/
https://ncbi.nlm.nih.gov/pubmed/33668802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules26051222
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