The case for using mapped exonic non-duplicate reads when reporting RNA-sequencing depth: examples from pediatric cancer datasets

BACKGROUND: The reproducibility of gene expression measured by RNA sequencing (RNA-Seq) is dependent on the sequencing depth. While unmapped or non-exonic reads do not contribute to gene expression quantification, duplicate reads contribute to the quantification but are not informative for reproduci...

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Vydáno v:Gigascience
Hlavní autoři: Beale, Holly C, Roger, Jacquelyn M, Cattle, Matthew A, McKay, Liam T, Thompson, Drew K A, Learned, Katrina, Lyle, A Geoffrey, Kephart, Ellen T, Currie, Rob, Lam, Du Linh, Sanders, Lauren, Pfeil, Jacob, Vivian, John, Bjork, Isabel, Salama, Sofie R, Haussler, David, Vaske, Olena M
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2021
Témata:
Technical Note
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7955155/
https://ncbi.nlm.nih.gov/pubmed/33712853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/gigascience/giab011
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