Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS

Current proteomics approaches disassemble and digest nucleosome particles, blurring the readout of the “histone code”. To preserve nucleosome-level information, we developed Nuc-MS which displays the landscape of histone variants and their PTMs in a single mass spectrum. Combined with immunoprecipit...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Schachner, Luis F., Jooβ, Kevin, Morgan, Marc A., Piunti, Andrea, Meiners, Matthew J., Kafader, Jared O., Lee, Alexander S., Iwanaszko, Marta, Cheek, Marcus A., Burg, Jonathan M., Howard, Sarah A., Keogh, Michael-Christopher, Shilatifard, Ali, Kelleher, Neil L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7954958/
https://ncbi.nlm.nih.gov/pubmed/33589837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-01052-9
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